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Archive: Published Articles


Interdisciplinary International Science Journal

Volume 41 (2023)
Issue 3B
Page No. 1788



Shankar C.,

Kumari  Anjani

Environment and Ecology 41 (3B): 1788—1798, July—September 2023
Article DOI:
ISSN 0970-0420

Morpho-Molecular Genetic Diversity Analysis of
Little Millet (Panicum sumatrense) using Yield
Attributing Traits and ISSR Markers to Evaluate
its Performance as a Summer Crop

Shankar C., Kumari  Anjani

Received 7 October 2022, Accepted 16 June 2023, Published on 21 August 2023



The knowledge of genetic diversity among the genotypes and its relationship plays a vital role for genetic improvement in crop breeding programs. The present study was conducted to assess the performance of 18 little millet genotypes in local conditions of Bihar and analyze the genetic diversity by using morphological traits and 16 ISSR markers. The field experiment was conducted in RBD design in the month of April 2020, to evaluate the performance of the plant as a summer crop. ANOVA analysis revealed the presence of substantial amount of variability among 18 genotypes. The maximum mean sum of square values was recorded for plant height followed by flag leaf length within the genotypes. The descriptive statistical analysis showed that plant height followed by length of flag leaf contributed the most towards the divergence. A total of 117 alleles including 43 unique alleles and 74 shared alleles were obtained by amplification with ISSR primers. All the primers were highly polymorphic and produced amplified product with a size ranged between 354-2315 bp. The primers UBC 807 followed by HB 10 and UBC 814 were found to be the most effective in detecting genetic diversity. The dendrogram based on Dice’s similarities coefficient, grouped the genotypes into five clusters. The genotypes WV-168 and WV-115 followed by WV-122 and WV-124 were found to have greatest variability. The present study identified genotypes which can be grown as summer crops. The diversified genotypes among the population were also identified, which could be utilized as parents for breeding program.

Keywords   Panicum sumatrense, ANOVA, Yield attributing traits, Dendrogram, ISSR markers, Summer millet.

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